Composite

Part:BBa_M36928:Design

Designed by: Alexandra Ritchie and Scott Platshon   Group: Stanford BIOE44 - S11   (2013-06-10)


Beta-glucosidase actuator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 108
    Illegal AgeI site found at 1015
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 608


Design Notes

The medium strength RBS was chosen so as to translate enough protein but not too much to have negative impacts in the cells. The strong terminator was chosen to ensure that transcription stopped at the location we wanted. The RBS and terminator were taken from the BioFab Registry, so they were standardized for functional and physical composition.


Source

This part is a composite of BBa_M36282, BBa_M36235, and the genomic sequence for the BglA1 gene from Shewanella frigidimarina, which is identified as BBa_M36926.

References